FastQCFastQC Report
Fri 27 May 2022
EGAF00005412797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157777798
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4012350.2543038406455641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3609460.2287685622282547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2933090.18590004659590953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2752580.1744592734143748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2716680.17218392159332835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2174710.13783371472835487Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1985150.12581935007104106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1927700.12217815335463106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1908450.12095808308847104No Hit
GCTCAACATCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1881830.11927090020612406No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1825810.11572033728091452No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1813800.11495914019537781No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACATCCACTACCTAAAAAATCCC1681180.10655364831495494No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1654980.10489308514750599No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG1594300.10104717014747537No Hit
GTTCAAGCTCAACATCCACTACCTAAAAAATCCCAAACATATAACTGAAC1587590.10062188851184246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)