FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005412801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141162274
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2860140.20261362465725086No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2674730.1894790955266136No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2666330.18888403568789208No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2394980.16966147768347795No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA2376570.16835730487028No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2352470.16665004985680523No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA2342740.16596077221028616No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2006930.14217183834825442No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2005060.14203936669368192No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1993000.14118503078237463No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1935930.13714216590191797No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC1862810.1319623116867613No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1829360.1295926984004239No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC1811770.12834661476195827No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1714270.12143967020536947No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA1681530.11912035364349542No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1668750.11821501260315485No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT1647790.11673019662463074No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC1636260.1159134061555285No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG1582980.11213902660706641No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA1562740.11070521575757557No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT1512330.10713414832067666No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1498410.10614804915936675No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1467370.10394916137437683No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA1456870.10320533657597497No Hit
GTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAG1427730.10114104565926729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1421960.10073229622243121Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)