FastQCFastQC Report
Fri 27 May 2022
EGAF00005412819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138168880
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4149450.30031726391644775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2906720.21037443453258073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2807000.20315717982225812Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2527580.18293410209303282No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2478820.17940508745529385No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2474480.1790909790974639No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC2208210.1598196352174238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2207740.15978561887452516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2080320.1505635711891129No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1896860.1372856174270212No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1888970.13671457711751012No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG1881740.13619130443845243No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1811560.1311120130669077No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1701720.12316232135629963No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1688880.12223302381838803No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1669150.12080506116862205No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1659910.12013631434227445No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1656880.11991701749337477No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC1653390.11966442805355301No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1571410.11373110934965963No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT1570780.11368551297513593No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC1482590.10730274429379466No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1475130.10676282531927594No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA1462880.10587622914798181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1426380.10323453443351353No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC1420040.1027756756803703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)