FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44684596
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1721090.3851640507167168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1661220.3717656975124045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1421650.31815214352614934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1365540.30559524360475365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1288470.2883476892126316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1120620.25078440901647625No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1063000.23788958503731356No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1051110.23522871282085664No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC915360.20484911623683474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT907460.20308116917964306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG866030.1938095177138896No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC839920.18796634079448765No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC784880.17564889699349637No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC783280.1752908317667234No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG764440.17107461372147126No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG762010.17053080215830976No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA737410.16502554929667484No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA728580.16304947682642135No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC711170.15915327957759762No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA689220.1542410722478055No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC684390.15316016284448447No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT679520.15207030181049416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA665970.1490379369212603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA642650.14381913624104378No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA626360.14017358465096114No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA597370.1336858903233678No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC596070.13339496232661474No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC587360.13144574474836923No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC577790.1293040671107332No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT566100.12668795304762295No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC546700.12234641217300028No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC541850.12126102695434463No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT538010.12040167041008941No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA499420.11176558472185806No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA499270.1117320161068481No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT493480.1104362675674633No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA488520.10932626536446699No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT482190.1079096698110463No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG475840.10648859844229096No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC472680.10578141961941426No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG471430.10550168116099784No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC467780.10468484486242194No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG467350.10458861483272669No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA460350.10302207946559482Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA455220.10187403283225387No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT450640.10084907112061615No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC447730.10019783998942275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)