FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005412849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100711872
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6740530.6692885224097512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6478680.6432886085167794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT5270600.523334528028632Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA2334100.23176016428331309Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA2024100.20097928474609228Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC1732980.17207306006584805Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC1718720.17065713960713588No Hit
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA1532430.15215981686846214No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1520640.15098915051444978No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT1280120.12710715971995834No Hit
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC1224550.12158943883001203Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1202660.11941591156204505No Hit
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT1085790.1078115199765128Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1048930.10415157410637743Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCC1040800.10334432071722388Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTA1021950.10147264465504126Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1011920.10047673426227248Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)