FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005412879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137170018
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2875400.20962306792144622No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2050070.14945467164697754No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1952290.14232629174110045No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1901540.13862650364309204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1848370.13475029215203574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1706950.12444045899301405No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1705300.12432017031593595No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1618860.11801850168161383No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1557940.1135772979194331No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1515750.11050155289766019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1503200.10958662992958125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1503020.10957350752844547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1479450.10785520200194186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1424870.10387619836865518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)