FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005412883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135108293
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2829640.20943496044317575No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2028680.1501521449908334No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1924610.1424494349876806No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1887890.13973161514223262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1808640.1338659500346141Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1687160.12487464407532704No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1684380.12466888320467494No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1594060.11798387534953166No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1538540.11387457911262337No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1501860.11115971985524234No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1479640.10951511318405896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1463940.10835308236778625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1424360.1054235804755523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1419840.10508903402398845No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1354120.10022478783001129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)