FastQCFastQC Report
Sat 25 May 2024
EGAF00005412917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152855388
Total Bases13.9 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2938510.19224117896321719No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2303140.15067444007927283Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2177610.14246210280791674No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2051590.13421770909377428No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2019620.13212618975524762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2001100.130914587060549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1953460.12779791576597876Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1872340.12249093895205054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1648740.10786273363160741No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1622310.10613364836050136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1576660.10314716547643056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1557110.10186817883056894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)