FastQCFastQC Report
Thu 12 May 2022
EGAF00005412927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53669251
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1832950.34152703193118905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1699220.3166095982967976Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1482630.2762531565793605Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1296950.24165606484800767Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1163790.21684483727935758No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1134010.211296036160445No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1112890.20736082193507788No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC977310.18209868440310448No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG880580.16407532872035052No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC863770.16094318141313357No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC822620.1532758487723259No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC819050.15261066341320842No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG805720.15012693208630767No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC783600.1460053914298152No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG783390.14596626287927886No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC752520.14021436595043965No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA742220.13829520370984869No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA731730.1363406394473439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA724020.13490406266336752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG714070.1330501146736704No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT699600.13035397121528675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA680770.12684544451719665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA677330.12620448159412548No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA675290.12582437567462978No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA673080.12541259426184279No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA624090.11628446240101245No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC615630.11470814079369208No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT615140.11461684084244067No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC603060.1123660175544466No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC589230.10978912301198315No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC584670.10893947448605162No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC582680.10856868488811218No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT559520.10425336474324935No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT547200.10195782311178518No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT536970.10005170372137298No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT536900.1000386608711942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)