FastQCFastQC Report
Fri 27 May 2022
EGAF00005412945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214017601
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA9928980.4639328706427281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6055600.28294869074810347Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5880720.27477740020083674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT5850430.27336209604554906No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT5731300.2677957314361261No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA5726830.2675868701098093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5456380.2549500589907089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT5081200.23741972511877657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC5001030.23367377153246383No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC4909840.22941290702534323No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG4800800.22431799896682328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4788420.22373954187067074Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC4491650.20987292535813445No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG4453840.20810624823329368No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA4401490.20566018773381164No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC4219250.19714500023762066No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA4172740.19497181449108944No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA4166560.19468305319430246No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT4045600.18903118159893775No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC3944410.1843030658025178No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG3820420.17850961706649537No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA3800640.1775853940162613No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC3510760.16404071364205228No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT3481290.16266372409248714No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC3458580.16160259641448835No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC3431380.16033167290759417No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA3431020.16031485186117939No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC3402290.15897243890702242No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA3369670.157448265201328No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC3254580.15207067011278197No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT3057530.14286348345713865No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC3001640.14025201600124468No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG2936700.13721768612853483No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT2867630.13399038147334433No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA2826470.13206717516658828No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC2815720.1315648800305915No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA2811690.1313765777610039No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA2802730.13095792060579167No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA2740740.1280614298634251No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC2719870.1270862764226574No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG2710940.1266690210213131No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA2685810.1254948185313039No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT2679940.12522054202448515No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT2665680.12455424168594434No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC2644240.12355245492168655No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA2635330.12313613402292085No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2618790.12236330039041975No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2452420.11458964069034677No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA2423320.11322993943848572No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA2409540.11258606716183124No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA2385370.11145672079559475No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT2384350.11140906116408622No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT2347650.10969424893235767No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC2346020.10961808697220188No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA2322600.10852378445266285No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT2312920.10807148520462109No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA2300280.10748087957494674No Hit
CAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGT2277230.10640386535311176No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA2239630.1046470005053463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2206780.10311208001999798No Hit
GTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAG2197780.10269155385962858No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC2181400.10192619624775627No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT2164090.10111738426597913No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG2146360.10028894773005141No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC2143560.10015811736904762No Hit
CTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACC2143200.10014129632263283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)