FastQCFastQC Report
Mon 13 May 2024
EGAF00005412959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95707074
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6412390.6700016761561429Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5514220.5761559485143177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4933080.5154352540335733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA1953950.20415941250068936Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA1922370.20085976089917867Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC1623850.16966875405677956Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1493290.15602712919632253No Hit
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC1460990.15265224804594904No Hit
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA1456640.1521977361882362No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT1190340.12437325165744803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150390.12019905655040714No Hit
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC1034570.10809754773194719Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1031470.10777364272989895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT1029770.10759601740619508Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTA969530.10130181181800627Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)