FastQCFastQC Report
Thu 2 May 2024
EGAF00005412961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47066425
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1189170.2526578128676652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1157370.24590140423879653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1042910.2215825824884724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC906650.19263200891081064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT709750.15079751648866468No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT680520.1445871446577895No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA679370.14434280912561342No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC574230.12200416751431621No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC536660.11402183191096414No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG520250.11053527009965171No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA500390.1063157016918111No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC490950.10431002567116579No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA488640.10381922995001214No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC473050.10050688999642526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)