FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005412965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41660876
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1050020.2520398274870648Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1005170.24127433134147253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA917630.2202618111054602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC795670.1909873426569331Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT634780.15236837554736007No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT609220.14623312289448737No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA600400.14411602866920034No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC509120.12220578367099146No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC477610.11464233253280609No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG461210.1107057854472383No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA448910.1077533751330625No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC435170.10445531678210511No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA431730.10362960202757138No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC424350.10185815583906589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)