FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005412977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158104927
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA6734350.4259418177398102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6677530.4223480018431051Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT5011350.3169635567397593Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA4494710.28428652321505454No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT4420890.27961747200958514No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT3980000.25173156052246237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3830300.2422631648917557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC3683690.2329902090907009No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA3593790.22730411178141208No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC3547350.2243668219144113No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC3452020.21833728179767603No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG3401380.21513434556027466No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA3308660.20926988568800262No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG3164780.20016960002770817No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA3013970.19063099785625276No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG2962770.187392642102798No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2949510.18655395856196183No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2934110.185579921870493No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2894420.18306956367020744No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2820680.1784055723956028No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2610380.1651042791348305No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2591790.16392847770012886No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA2575780.16291585903581612No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT2502570.15828538980319065No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC2496890.15792613471179176No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC2464530.15587939267699102No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC2434230.15396294386195822No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC2372450.15005541225163718No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2370200.14991310169606542No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC2333480.14759059342913458No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC2331570.1474697875797381No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG2290280.1448582307621571No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT2288080.14471908266337583No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC2203710.13938275307511447No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA2157960.13648910511182236No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA2112070.13358660226951688No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC2067260.13075240849388584No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT2043530.12925150650112252No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2022540.12792390714047766No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA2020220.12777716914539924No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC2016270.12752733505895106No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA2015350.12746914585400618No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2010760.12717883232063984No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA2008820.12705612899716906No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC2006050.12688092889097632No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG1971890.1247203384117182No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT1957730.12382473064865335No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA1956920.12377349884864752No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1935280.12240478754972639No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA1918250.12132765476688782No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC1907420.12064266662606916No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1880390.11893304248513394No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1813740.11471748758341983No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA1790370.113239355279548No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT1788450.11311791693879344No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA1785370.11292310960049967No Hit
CCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGC1778780.11250629779551398No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG1747250.1105120525434353No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG1744860.1103608871088502No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC1738070.10993142547670257No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA1726050.10917117086427042No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA1717420.10862533082223302No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1703270.10773035555052626No Hit
GTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAG1702990.10771264579249955No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA1695180.10721867004182609No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT1680320.10627878788369448No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT1673270.10583288147623636No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA1652590.1045248893476925No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTT1647840.1042244559525966No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC1630630.10313593832531227No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA1619490.10243134295239262No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT1604510.10148387089796386No Hit
GTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACAC1603100.10139468961647222No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA1599260.10115181293496311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)