FastQCFastQC Report
Fri 27 May 2022
EGAF00005412979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156949334
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4921420.3135674344435256Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3636870.23172255066721087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3003360.1913585692564965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2598790.1655814608299007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2193390.13975146909511574No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2184860.13920798160252149No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1963650.12511362424768238No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1922410.12248602469380342No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1769140.11272045283097537No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG1693830.10792208904817652No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1667760.10626104345240484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)