FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005412981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155824642
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA6610720.42424098750696954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6557070.42079801473248374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4853560.31147576774153607Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA4449470.28554341231857283No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT4376850.28088304544283826No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT3935130.2525357959750679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3705470.23779743386158395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC3624300.2325883732818074No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA3553030.2280146422540794No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC3518690.2258108829795996No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC3403600.21842501650027857No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG3366270.2160293748661396No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA3273150.21005342659474874No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG3097270.19876637996704014No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2975600.19095824394706454No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG2932100.18816664440018416No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2913770.18699032210836075No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2898890.186035402539221No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2858760.18346007173884604No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2786350.17881318155057915No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2576590.1653518960114152No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2561100.16435782987391684No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA2556250.16404658256811527No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC2462540.15803277122241038No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT2458790.1577921160890586No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC2429040.15588291869780135No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC2394200.15364707207220793No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC2333680.1497632190934217No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2332470.14968556770372685No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC2303890.14785145471407532No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC2294200.14722960184949438No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG2251290.144475865376928No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT2238780.14367303985206653No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC2162400.13877137609595794No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA2142340.13748403156928157No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA2077210.13330433321322824No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC2033240.13048257155630108No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT2016620.1294159880052861No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2013720.1292298813688274No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1998490.1282525006539081No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA1997310.1281767745052801No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA1996700.12813762793692154No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA1995410.12805484257104857No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC1992000.12783600683645402No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1977430.12690098142500464No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA1931050.12392455873571011No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG1930880.1239136490363315No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT1925140.12354528624554774No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1911970.12270010541721636No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC1894100.12155330348841745No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA1889570.12126259208732854No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1870490.12003813876883478No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1777370.11406219049744393No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA1775250.11392614012872239No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT1758490.1128505721193956No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA1756860.11274596735476536No Hit
CCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGC1752660.11247643360541139No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG1737980.11153434897671703No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG1718590.11029000150053288No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA1698050.10897185311678753No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA1694550.10874724165899255No Hit
GTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAG1693680.10869140966805495No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC1689900.10844882929363638No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1675620.10753241454583287No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA1675110.10749968544769703No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT1652560.10605254591247514No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT1637090.10505976326902135No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA1630900.10466252186223537No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTT1620600.10400152242929588No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC1605720.1030466028601561No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT1590860.10209296678506087No Hit
GTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACAC1590550.10207307262737045No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA1584290.10167133899142858No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA1571570.10085503677909943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)