FastQCFastQC Report
Thu 16 May 2024
EGAF00005413027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75831075
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2645230.3488319267529835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2386190.31467178857744005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2143240.28263347183196336Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1943090.25623927921369966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1700500.2242484364094799No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1697480.22385018279115787No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1473640.19433194109406995No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1447660.19090590500002802No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1396230.1841237250032391Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1335830.1761586526367983No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1333150.17580523551855223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1329630.1753410458707067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1303750.17192819698256948No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1269710.16743927209260848No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1265150.16683793550335402No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG1242310.16382597767471976No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1215980.16035378636001138No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC1153690.15213947580197168No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1153410.1521025516254385No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1142970.1507258073289875No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1117090.1473129584408503No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1108330.14615775920359828No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1089970.14373658819949472No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1080320.14246402282969084No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT1075770.14186400496102686No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC1010050.13319737324045583No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC997530.131546335061187No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT986380.13007596160281257No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT970790.12802007620226932No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA965520.12732511045109146No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC952490.12560681752170863No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA944330.12453074151988482No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC937220.12359313118006042No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA926640.12219792479534279No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC919610.12127086422024218No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC914930.12065370298390204No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG906140.11949454758487864No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC892980.11775911128781968No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC866960.11432780031141586No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC827060.10906610515543924No Hit
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC819730.10809948296262449No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG816010.10760891890296953No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA815980.10760496274119813No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA814380.10739396744672287No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT812730.10717637854929526No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA796950.10509543745753308No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT793730.10467080942740163No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA792510.10450992551536424No Hit
CCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGC791160.10433189823565074No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA790510.1042461813972702No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA764450.1008095955385045No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA761730.10045090353789655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)