FastQCFastQC Report
Fri 27 May 2022
EGAF00005413029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167988035
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5792930.344841821621403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4835660.28785740603490007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4448350.2648015973280478Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4124010.24549426987463718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT3801540.22629825987309157No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA3789580.2255863044055489No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT3299700.19642470369987958No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC3189150.1898438778690399No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA3013920.1794127778207537No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC2944640.17528867457732927No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2921220.17389452766680677No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2857590.17010675790094218No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA2837830.1689304836502195No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2771510.16498258343220695No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2713960.16155674420502628No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2706000.16108290093398617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC2611090.15543309379147152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2600690.15481400208056484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2549060.15174056890420798No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2519450.14997794336959772No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2499590.1487957163139625No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG2495200.14853438817830092No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC2462290.14657532008157606No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2407770.14332985084324606No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2399860.14285898397466223No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2231530.132838627465343No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC2223380.132353473864969No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA2183430.12997532830239963No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT2164830.12886810658866268No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC2124310.1264560300380917No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT2114070.12584646281504513No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2064740.12290994415167723No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA2064710.12290815831020346No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC2056170.1223997887706705No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG2053340.12223132439164491No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC2041110.12150329635083831No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC2018450.12015439075765129No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC2006950.1194698181927064No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC1927720.11475341086048182No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1899770.11308960188742014No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA1860740.11076622213004636No Hit
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC1845520.10986020522235408No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC1845140.10983758456368635No Hit
CCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGC1813170.10793447283313957No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA1811390.10782851290569594No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT1774460.10563014205148599No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA1767770.10523189940283545No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT1766400.10515034597553333No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA1762750.10493306859622473No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG1752450.10431992969023061No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA1736500.10337045730667664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)