FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005413033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103522743
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1965310.18984330815113737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1487620.14369982449170612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1437560.13886417209791282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1348160.13022838855805818No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1322230.1277236249429751No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1291170.12472331804422919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1155550.11162281509484347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1146480.11074667911378662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1103360.1065814108113422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1077900.10412204784797868Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1074640.10380714119988108No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1043690.10081745998558017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)