FastQCFastQC Report
Tue 31 May 2022
EGAF00005413039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161805441
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3827220.2365322189628963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3611790.22321808078135025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3492550.21584873650818703Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3344180.2066790819475595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2712070.16761302853839136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2470000.15265246859034856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2218630.13711714428688465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2194400.13561966683184654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2109250.13035717383570555No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2081430.1286378249789511No Hit
GCTCAACATCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2020730.12488640601399802No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2003300.12380918636722482No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1776420.10978740819970324No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACATCCACTACCTAAAAAATCCC1726200.10668368068042904No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1655420.10230929131734204No Hit
GTTCAAGCTCAACATCCACTACCTAAAAAATCCCAAACATATAACTGAAC1629250.1006919167817107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)