FastQCFastQC Report
Sat 25 May 2024
EGAF00005413069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222263723
Total Bases20.2 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8974330.4037694446430199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA8757340.39400671786641495No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT6652050.29928635722528596No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA6444130.2899317042394723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT6168440.2775279706801276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA5471610.2461764756815488No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA5462690.24577515063040675No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC5313890.2390804008983508No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT5250110.23621083679948976No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC5170180.2326146583983928No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG5059590.22763903761298912No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC4960870.22319746709182947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4840470.21778047873336487Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC4604430.2071606620213052No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA4536450.20410213321226514No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC4374870.19683239086209312No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG4340190.19527208225518655No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG4329620.19479652106790274No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT4278880.1925136474025498No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC4168010.1875254289698009No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT4122490.18547741144424185No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA3990140.17952277349372034No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA3693450.16617421638348062No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC3621390.16293212185598097No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA3614030.16260098369719111No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC3518330.15829528780096966No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC3498510.15740355433531544No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC3460790.1557064712715174No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC3427460.15420690132145407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3325320.1496114595362915No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT3298050.14838453866805784No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC3293920.14819872336971518No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC3260630.14670095308355832No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT3243430.14592709760377764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3205910.14423901285951193Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA3204610.14418052378255178No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA3173630.14278668408699335No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA3078330.13849898482983658No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA3045440.13701921118274438No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA3011490.13549174644213083No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT2995330.1347646822239183No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT2921600.13144745172832367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2899260.13044233943656203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC2882620.1296936792514719No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2866470.128967064949236No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG2803070.12611459765748637No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA2797570.1258671438703472No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG2784770.1252912514202779No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG2755270.12396399928925875No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2741140.12332826801429939No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT2695800.12128834897631945No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA2592850.11665646399705093No Hit
GTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAG2580980.11612241373280695No Hit
AGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTC2560500.11520098581269604No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA2552630.11484690193909872No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC2548050.11464084042180829No Hit
ATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTT2518880.11332843551801748No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA2482560.11169434069094578No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC2477550.11146893278666083No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA2469230.11109460269411577No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC2441760.10985868350634978No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT2425740.1091379181118099No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA2391050.1075771595889267No Hit
GTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGA2361110.10623011115493643No Hit
CCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAAT2311320.10398997950736208No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA2281690.1026568784686469No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA2264340.10187627424921701No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2262340.10178629105389365No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA2261800.10176199559115637No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA2259260.10164771693309572No Hit
GGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC2252110.10132602700981483No Hit
AGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATA2244160.10096834380840458No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC2228900.10028177202808755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)