FastQCFastQC Report
Mon 4 Jul 2022
EGAF00005413071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223961719
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9345370.4172753290931831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA9002240.4019544072172442No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT6783900.3029044441295791No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA6599940.2946905403954325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT6486290.28961601245791474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA5553100.24794862375565174No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA5531390.24697926166569567No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC5450830.24338221836920265No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT5354880.23909800406559661No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC5297220.23652345693953172No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5166020.23066531294127102Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG5147650.22984508348053895No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC5049960.22548317732817544No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC4729020.21115304977633254No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA4634150.20691705799954144No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC4455780.19895275049214997No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG4451960.19878218562878594No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG4416840.19721406049754425No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT4384270.1957597941101711No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC4279100.19106390230912632No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT4193540.1872436065736752No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA4064770.18149396326074813No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA3796910.16953388360088448No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA3684410.1645107037243271No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC3667820.1637699521318641No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC3603960.16091857198149118No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC3596480.16058458633280986No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC3553180.15865122021143266No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC3509310.15669240331201423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3473010.1550715906051784No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC3396020.15163394955010145No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT3392970.15149776556233702No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC3338050.1490455607728212No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT3327450.14857226560223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3299440.14732160543918668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA3271640.1460803218785796No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA3260360.14557666437629013No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA3120270.1393215775415619No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA3114300.13905501412944593No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT3078360.13745027559821507No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA3068360.13700377072029887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3020230.1348547427428881Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT2980920.1330995320677995No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2937600.13116527293666647No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC2915310.13017001356379124No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG2886860.12889970718611962No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG2846860.12711368767445475No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG2843340.12695651795742827No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA2838900.12675826979163346No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2772320.12378544031446731No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT2732740.12201817400767495No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA2674220.11940522746210927No Hit
AGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTC2628310.11735532356759594No Hit
GTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAG2612550.11665163188No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA2604420.11628862341425412No Hit
ATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTT2601450.116156011465513No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC2577070.11506743257315326No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC2535440.11320863276638808No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA2534030.11314567557860189No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC2528910.11291706508110881No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT2490720.1112118629523468No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA2488600.11111720391822853No Hit
GTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGA2432880.10862927873847941No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA2432280.10860248844580445No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA2363980.10555286012963672No Hit
CCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAAT2358690.10531665904921902No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA2342720.10460359075918685No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA2332410.10414324423005522No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2318760.1035337650716996No Hit
GGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC2312150.10323862534739699No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC2312070.10323505330837365No Hit
AGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATA2307990.10305287931818383No Hit
CCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAG2304530.10289838863042483No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA2302750.10281891076215574No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTT2282400.10191027333559625No Hit
GTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACAC2246100.10028946062876039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)