FastQCFastQC Report
Fri 27 May 2022
EGAF00005413073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165929003
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA6482430.3906749201644995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5737770.3457966899252688Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5650580.34054203290789375Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4848750.29221835317120537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT4486050.27035960675301596No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA4285230.25825684012577355No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT4232380.25507174294297424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3743210.22559106197968296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC3531590.21283741456579477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3507030.2113572634435705Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG3386160.20407282263969245No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC3300850.19893146709258538No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC3095260.18654122811790774No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG3085490.1859524220729513No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC3041020.18327236016719753No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA2978550.17950749695036738No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA2930380.17660444810844791No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2837590.17101229734984907No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2754870.166027032658058No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2726720.1643305239410135No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2701750.16282566345559252No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2680140.1615232992149058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2662690.1604716446105567Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2589310.15604927126573526No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA2347450.14147315764923868No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC2329850.14041246303396399No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC2312740.13938129912104635No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2300100.13861952753371273No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC2227910.1342688716089013No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT2219350.13375298831874496No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC2170870.1308312567875792No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT2163320.13037624290432215No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC2016900.12155198690611069No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT2010440.12116266376891326No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC1989020.11987175020873235No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA1958380.11802517731032229No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA1942220.11705126679993372No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1912180.11524085394522621No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC1883320.1135015558431337No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC1871080.11276389095160175No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA1858560.11200935137300862No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT1825570.1100211516367636No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1823200.10987831946413854No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC1806020.1088429368794556No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG1785220.10758938869776732No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA1748910.10540110338636821No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA1744380.10512809505641398No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1734540.10453507033969221No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA1706490.10284458829659816No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA1676270.10102332742877988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)