FastQCFastQC Report
Fri 27 May 2022
EGAF00005413111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173234950
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5488760.3168390674052782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5477420.3161844650862889Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT5308300.3064220008722258Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4720700.2725027484350011No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA3395900.19602857275624808No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT3370730.19457563268843844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3164680.1826813815572435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT3038520.1753987864458067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3033240.1750939980644783Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA2887510.16668172328967104No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2840280.16395536812865996No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA2805410.16194249486030388No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC2662270.15367972802254973No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC2636660.15220138892296273No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2539880.14661475643338714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2499660.14429305402864723No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2442550.14099637515408986No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2378200.13728176675665044No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2375430.13712186830659748No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2290480.13221812342139966No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2248980.1298225329242165No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG2247030.129709969033385No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2125780.1227108040265547No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC2060820.11896098333506028No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT2033820.1174024063850857No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1873240.1081329142877924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1838630.10613504953821384Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1830990.10569402998644327No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC1820720.10510119349473072No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC1819600.10503654141384287No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA1791200.10339714936275851No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC1782680.10290533174743319No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC1766530.1019730718310595No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC1766250.10195690881083753No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT1753190.10120301936762761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)