FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005413119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61042786
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2080860.3408854897284669No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1965110.3219233801026054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1691220.2770548513300163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1484050.24311636103896045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1311580.2148624081476229No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1269250.207927927798053No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1248480.20452539633430228No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1114450.18256866585348838No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG984690.1613114447299309No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC984090.16121315301696748No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC936180.15336455973683769No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG933330.15289767410026142No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC932930.15283214629161912No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG884940.1449704474497609No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC874150.14320283481163523No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC854200.13993463535560122No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA840110.13762641829617672No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA826850.13545417143968494No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG823900.1349709038509481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA800600.13115390899753496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT794130.13009399669274596No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA768590.12591004611093604No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA764010.12515975270198185No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA761840.12480426434009746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA742940.12170807538174945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC703110.11518314383619384No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT700710.11478997698434013No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA696740.11413961348356544No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC671300.10997204485391607No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC670890.10990487885005773No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC667570.10936099803832676No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC663740.10873356927057687No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT637210.10438743736237727No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT622420.10196454663782875No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT613330.10047542718643279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)