FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005413131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88120492
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3871590.4393518365739492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3814060.4328232756576076Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3404480.3863437348942627Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2324150.26374682519929643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1542530.17504781975116526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1520600.1725591818075641No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1454300.16503539267574674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1382530.15689086257030885Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1273840.14455661459538832No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1273670.14453732282838366No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1258870.14285780428915446No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1224920.13900512493734146No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1205710.1368251552658149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1204600.1366991913753727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1198820.13604327129721427Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1122640.127398290059479No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1102180.125076469159977No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA1099800.12480638442191176Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG1098600.12467020724305534No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1059420.12022402235339312No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1043010.11836179943253153No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG973340.11045557939009239No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT960250.10897011333073356No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC954630.10833235020975597No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA945090.10724974163784742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG913260.10363764196868079No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC911880.10348103821299591No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC909570.10321889714369728No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT885710.10051124090410207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)