FastQCFastQC Report
Mon 4 Jul 2022
EGAF00005413135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188134117
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT7225120.38404092331642325Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5354630.2846177017430603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4474550.23783830765793532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3942790.20957336515417882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3463990.184123435729629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA3112590.1654452711519623No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT3041310.16165648466620225No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT3026130.16084961347016075No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2916110.15500165767381788No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2704440.14375064146392968No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC2608120.13863088957969277No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2591450.1377448195640135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC2519230.1339060687222403No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2465540.13105225353676814No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2427760.12904411165360297No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2425360.12891654308505884No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC2423870.12883734426542104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2347910.12479979907099997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA2330240.12386057548509397No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA2207500.1173365062754673No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2177530.11574349377577273No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC2135170.1134919085409692No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC2112590.11229170092525004No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT2106860.11198713096785097No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2096580.11144071226592038No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2094380.11132377441142162No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2023680.10756581699639305No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC1986320.10557999961272309No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA1967410.10457486559973594No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1959040.10412997021693837No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1938870.10305786270546559No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC1915910.10183745673306027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)