FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005413137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36834561
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2116030.5744686355838474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1777720.4826228280554233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1175210.3190509043938382Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA1148080.31168553902407037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1098490.2982226393304918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC884050.24000557519879223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA668600.181514312061436No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA636400.1727725219801045Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT636190.17271551030566104No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT619980.16831475200695345No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC590120.1602082348694206No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC570330.15483556326353395Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC558630.15165919854454082No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC532450.1445517431305887No Hit
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA517620.1405256329782239No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG509610.13835104482445168No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC486210.13199831538646545No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG485150.13171054217260794No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC479510.13017937148755487No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT476060.1292427511216979No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC454480.12338412286222171No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA445390.12091633181131167No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA441530.11986840293820794No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT437160.11868201714145582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA437110.11866844293325499Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA420860.11425682526798678Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA413900.1123672954864319No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG409350.11113204254015678No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA400970.10885700524569845No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT398790.10826516976814247No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC388500.10547159772041263No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC383040.1039892941848825No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC381740.10363636477166106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)