FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005413155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118900789
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5069660.42637732202096656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4140700.3482483198660692Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3581300.3012006926211398Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3530350.2969156075154388Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3493630.2938273185050101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT3273850.2753430004572972No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA3178680.2673388483570113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3097500.26051130745650475Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT3094730.2602783401210231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3084100.2593843174581457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC2698290.22693625691583932No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2468500.20761006051860598No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2426960.2041163915236929No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC2310190.19429559882903719No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2269630.19088435149072056No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG2259130.19000126231290188No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2209370.18581626064735365No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA2124800.1787036080980085No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA2096480.17632179042983476No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2075040.17451860643246025No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2057440.17303838076297373No Hit
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2010640.16910232614183912No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1976030.1661914960042864No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1910880.16071213791524966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC1843780.15506877755033233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1790610.15059698216132106No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT1757450.14780810243403852No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC1740960.14642123190620712No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA1727020.14524882589298882No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC1685830.14178459320400305No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC1669530.1404137023851036No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC1641660.13806973139597922No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT1579890.13287464391846887No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC1569840.1320294014196996No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT1504010.1264928527934327No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1495610.12578638145117776No Hit
AAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAA1468680.12352146796940094No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG1412270.11877717649123422No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC1404790.11814808058170244No Hit
TTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT1392950.11715229240404788No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA1374360.11558880404065273No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC1354290.1139008421550508No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1353970.11387392896106013No Hit
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA1351330.11365189511063715No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC1332110.11203542139657291No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC1311360.11029026897374078No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC1303110.10959641319116897No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1300120.10934494303481872No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA1283670.10796143665623614No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA1258710.10586220752496436No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA1253830.10545178131660674No Hit
CACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCA1251260.10523563472736921No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA1250890.10520451634681752No Hit
CACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCT1245440.10474615101166401No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA1229080.10337021396889133No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1227220.10321378102882059No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT1219610.1025737516342301No Hit
CAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGT1212230.10195306609782043No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC1210120.10177560722494448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)