FastQCFastQC Report
Sat 25 May 2024
EGAF00005413175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157325078
Total Bases14.3 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4194910.26663962626479676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3520490.2237716990040202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3153900.20047026450544647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3065910.1948773862994684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2790820.1773919349336029Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2452400.15588106048801706Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2152670.13682942524903755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1899750.12075315799303146No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1848120.11747141800241155No Hit
GCTCAACATCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1807690.11490157977229797No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1740640.11063970360783804No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1721620.10943074186812098No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACATCCACTACCTAAAAAATCCC1634700.10390587570533415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)