FastQCFastQC Report
Fri 27 May 2022
EGAF00005413177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158842670
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4194150.26404428986241546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3510830.22102562239730675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3172440.19972215274396987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3001390.18895363569499304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2794270.1759143182370329Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2413990.15197364788693116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2132660.1342624119828759Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1898860.11954344509570382No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1846700.11625969268836893No Hit
GCTCAACATCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1806630.11373707077575565No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1739340.1095008035309404No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1726150.10867042212272056No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACATCCACTACCTAAAAAATCCC1630840.1026701452449773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)