FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005413183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113420507
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2920160.25746314112314805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2544410.22433421144908125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2223240.1960174626974644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2154170.18992773502590674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1537170.13552840140275516No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1470880.12968377931867295No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1412290.12451804681141128No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT1400710.1234970674218552No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG1299870.11460625898983152No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1177820.10384541835983857No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1164390.10266132913689055No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1156730.1019859662591704No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1149870.10138113736345757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)