FastQCFastQC Report
Mon 4 Jul 2022
EGAF00005413189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206961121
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT14594690.7051899375825278Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT11864320.5732632265748115Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA7849760.37928669704103507Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA6888510.33284077544206964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4101330.19816910442807273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA3847320.1858957847450005Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT3599660.17392928597444154No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC3337910.16128198300588062No Hit
AAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACC3318750.16035620526040734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3290860.15900860915804568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3281250.15854427073769087No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC3149580.1521822062415288Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC2981390.1440555591115106No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC2962300.1431331636438131No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC2929430.14154494263683468No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT2825720.13653385652081002No Hit
ATTTTTCGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2665100.12877297857311085No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG2467540.11922722432490111No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2399450.11593723441418739Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA2383870.11518443601781611No Hit
CAACATTTTTCGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGC2319220.11206066090065293No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC2256260.10901854363264682No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA2144680.10362719285812141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)