FastQCFastQC Report
Fri 27 May 2022
EGAF00005413225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165945443
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA5248900.3163027501755502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5128540.3090497640239509Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA4038730.24337697540751388No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT4029650.24282980762538928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3938780.23735391155031596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT3788110.2282744214916465No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA3391590.20437982138503194No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG3215820.1937877860255554No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA3203530.19304718117508052No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC3153620.19003956619646376No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC3116510.1878032890604896No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC2992900.1803544554097819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2843250.171336431335448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG2759590.16629501540455074No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA2726920.1643262960827433No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC2714870.16360015381681797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2671960.16101436422089638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT2600530.15670993749433662No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA2540910.15311719044915262No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC2497640.1505097069764067No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2448270.1475346328130264No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT2336630.14080712056672745No Hit
AAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTA2285700.1377380396037751No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC2249230.13554032935993307No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2179100.1313142416330167No Hit
AAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAAC2160500.1301933913304266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2092340.12608601731835445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAA2039220.12288496527138743No Hit
CTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC1985720.11966101413221693No Hit
AATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC1982580.11947179531769365No Hit
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC1967450.11856004988338245No Hit
CTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCAC1959790.11809845239317598No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1930720.11634667183961177No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1912350.11523968151388164No Hit
AACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAA1911510.11518906246795822No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA1900520.11452679661712675No Hit
AAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGC1869970.11268583012550697No Hit
ATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAAC1843390.11108409888664432No Hit
CCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATT1823370.10987767829213604No Hit
CAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC1786980.10768478890981056No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA1786290.1076432089792306No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1751540.10554914725799369No Hit
GAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAA1730880.1043041597713533No Hit
GGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG1725780.10399682984967536No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1708970.1029838463235173No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1699680.1024240237799118No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT1690800.10188890815157846No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1661710.10013592238263512No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA1660120.10004010775999436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)