FastQCFastQC Report
Sat 11 May 2024
EGAF00005413243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35010167
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA978960.2796216310536308Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA939040.2682192290028208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA516950.14765710771959473No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT491190.14029924507358105No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT484080.13826840643176594No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA474600.13556062157601248No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG447480.12781430034309746No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA446880.12764292155475865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC431960.12338130235139981Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA426890.12193315158993673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA403820.11534363717830881Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC393700.11245304828166058No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA375350.10721171367163146No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG370370.10578926972841918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)