FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005413245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141589441
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3008120.21245369561138389No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2112610.14920674769808578No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2026760.143143442454865No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1974890.1394800336841502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1929780.13629406164545843Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1761560.12441323219857899No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1751650.12371332124971099No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC1666440.11769521711721426No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1622310.11457845927931871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1572930.11109091108001479Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1562900.11038252492288603No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1555230.10984081786155227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1548990.1094001070319926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1489430.10519357866523393No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1418480.10018261178105789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)