FastQCFastQC Report
Tue 31 May 2022
EGAF00005413273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168535796
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT7255770.4305180366549549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3983480.23635809688761908Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3340900.1982308850281278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3076890.18256596361285765Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2939600.17441991967095227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA2309520.1370343900117219Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2219100.13166935764791474No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2149070.12751415728917315No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2142650.12713322931111914No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2058610.12214675154232518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1928430.11442257643592818Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA1876020.11131285130667433No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1852300.10990543516345928No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1800840.10685207788142526No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1774520.10529039184055593No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC1770060.10502575963150286No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1738160.10313298665643707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)