FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005413297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157243623
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6759650.4298838878826902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT6475590.41181892635480677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5880900.37399926863806743Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4738350.3013381343928968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3218020.20465186050820008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2763230.17572922496195603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2593570.16493959821823745No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2589770.1646979349998823No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2426960.15434393800504076No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC2292280.14577888478186488No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2087050.13272716312317479No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2060220.13102089361042007No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2006760.12762107370166612No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1903420.12104910607408224No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1883080.11975557190004457No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1834020.11663557255991233No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC1755350.11163250798412346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1746440.11106587133266448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT1742080.11078859458739385No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1703590.10834080056779155No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT1697520.10795477537426112No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC1693420.10769403348077271Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA1671480.10629874637269074Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1631930.10378354103428411No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1626690.10345030017528914No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1619740.10300831086803439No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1592330.10126515591668858No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1588340.1010114095374157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)