FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005413307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185699298
Sequences flagged as poor quality0
Sequence length91
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCGCG33563051.8073870155394987No Hit
AGCCAGGATGGGCCCAGGCCTGGGATCCCCCACCCCAGGACCCCCAGGCC33403901.7988167085047353No Hit
CACTAGTATGAATGCACTGCACATGTCTGGATGTAGCCTAGCAGAGAGAG33222731.7890606134655394No Hit
ANNAGTGGTATCAACGCAGAGTACATGGGGAGACCGTGTGTTTCCAAAAT32621021.7566582292626653Clontech SMARTer II A Oligonucleotide (100% over 21bp)
CAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCC32300451.7393953745587127No Hit
ANGAATTTCAGGGTGAATGGACTGCTCCCGCTCCTGAGTTCACTGCTACT31737371.709073235161072No Hit
TGGTGCTTCTAACCTGATGGCACTTAGCTATCAGAAGACCACAAAAATTG30924331.665290624846627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3366350.18127962982391027Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)