FastQCFastQC Report
Thu 23 May 2024
EGAF00005413325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129672451
Total Bases11.8 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2173820.1676393083678198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2133860.16455769776419202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1719620.13261259324850735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1364950.10526137120674922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)