FastQCFastQC Report
Sat 25 May 2024
EGAF00005413339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177575678
Total Bases16.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2952980.16629417008336017Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2626340.1478997591100286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACAGGAAGGGGAACATCAC1783760.10045069347841658Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)