FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29164
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT2140.7337813742970786No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT740.2537374845700179TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT610.2091619805239336RNA PCR Primer, Index 6 (95% over 23bp)
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTG310.10629543272527775RNA PCR Primer, Index 40 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA608.440111E-1056.5911941
GTATCAA1101.8189894E-1242.4433941
GCTGGGG450.00164734637.7274638
TATCAAC1259.094947E-1237.350192
CGCAGAG1301.6370905E-1135.9136478
AACGCAG1451.8189894E-1235.125576
ACGCAGA1451.8189894E-1235.125577
TCAACGC1403.8198777E-1133.348384
ATCAACG1403.8198777E-1133.348383
CAACGCA1403.8198777E-1133.348385
ACGGGGG353.9285084E-430.3167116-17
GCAGAGT1551.2369128E-1030.121129
GTACGGG1500.029.71037914-15
CAGAGTA1450.029.27130710-11
GTGGCAC300.006797606628.29559718-19
GAGTACG1700.026.2150412-13
TACGGGA552.0021836E-423.15094214-15
CTTGAAA704.1018728E-521.25816256-57
AGTACGG1401.2732926E-1121.22169712-13
AGAGTAC1452.0008883E-1120.48991610-11
Produced by FastQC (version 0.11.5)