FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335827
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT24780.18550306289661758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8950.047.4876672
GGTATCA25000.045.3966561
GTATCAA53250.044.861041
GTGGTAT10250.041.8841931
TATCAAC58600.040.7607842
TCAACGC60600.039.2007374
ATCAACG61850.038.5459023
CAACGCA62400.038.1380545
AACGCAG65300.036.4443246
CGCAGAG68000.034.9347768
ACGCAGA68300.034.8435487
GCAGAGT70200.033.779429
CAGAGTA59700.027.19218410-11
GAGTACG70200.023.12584312-13
AGAGTAC57750.022.9592510-11
GTACGGG73150.021.90273314-15
GTGTAAG3200.021.2531171
AGTACGG66700.019.68810812-13
ACGGGGT10050.019.45194816-17
ACGGGGG9150.019.27517516-17
Produced by FastQC (version 0.11.5)