FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449568
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.2324140709507936No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.17494867436367248No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.11272323892359655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12550.047.7563671
GTATCAA56550.044.4232941
TGGTATC13850.043.5747152
TATCAAC60900.041.4538732
TCAACGC64700.039.3462684
ATCAACG66000.038.5068743
CAACGCA66250.038.4257135
AACGCAG68650.037.2680746
GGTATCA27000.036.2092861
ACGCAGA73150.034.9149327
CGCAGAG74550.034.259258
GCAGAGT79400.032.1665889
CAGAGTA57000.025.46049710-11
AGAGTAC54700.021.94735310-11
GAGTACG66200.021.76169612-13
GTACGGG71650.019.95813414-15
ACGGGAT12500.019.54829416-17
CGTAGCG550.005484086519.31649812-13
CCGTCTT16250.018.831484-85
GACAGGT8200.018.6614151
Produced by FastQC (version 0.11.5)