FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212888
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.17849958116495504No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.12490848289372143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9400.048.8531041
TGGTATC10500.043.3123362
GTATCAA40000.042.6265951
TATCAAC45600.037.6559562
TCAACGC46650.036.530554
ATCAACG46950.036.3876383
CAACGCA46950.036.2971235
GGTATCA20600.036.1216131
AACGCAG49800.034.3052146
CGCAGAG52950.032.102558
ACGCAGA53650.031.7615957
GCAGAGT57600.029.7322719
CAGAGTA42100.024.83124710-11
AGAGTAC39900.022.4726910-11
GAGTACG47900.021.78018412-13
GTACGGG52400.019.70659314-15
AGTACGG45650.019.50243412-13
CTTAGCG1801.3521532E-518.8877813
GATCGAC3000.018.41482720-21
TCGTGCA1902.1545327E-517.892959
Produced by FastQC (version 0.11.5)