FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212888
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22150.18262197333966534No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA300.00192489936.66963635
GTATCAA40000.030.8164771
GGTATCA18050.028.6579821
TGGTATC7450.028.425122
TATCAAC44700.027.6868062
GTGGTAT7850.026.6384471
TCAACGC45950.026.6233864
CAACGCA46200.026.4198135
ATCAACG46300.026.3649273
AGAGTAC46100.025.70825411
AACGCAG47350.025.6060856
CGCAGAG48700.024.8398138
ACGCAGA49200.024.6991377
CAGAGTA48550.024.6920810
AGTACGG51250.022.91216313
GCAGAGT53450.022.7370959
GAGTACG53550.022.18301212
CGTATTA650.00323987921.15381615
TACGGGG20800.020.22833615
GTACGGG58650.019.83372914
Produced by FastQC (version 0.11.5)