FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100561
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31780.28876182237967724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7400.035.671372
GTGGTAT8550.032.1803361
GTATCAA43350.031.4175851
TATCAAC47500.028.6544382
CGCACGG507.032949E-427.49918447
TCAACGC49750.027.2393024
ATCAACG49900.027.2137453
CAACGCA50600.027.1064385
AACGCAG51850.026.6650056
AGAGTAC49300.026.54972611
GGTATCA18550.026.4017561
CAGAGTA51100.025.98647310
CGCAGAG54100.025.5571778
ACGCAGA54650.025.4005647
GCAGAGT56050.024.6713969
GAGTACG57600.022.58179512
AGTACGG57200.022.50035313
TACGTAG650.003238513821.1551414
TACGGGG22900.020.89742315
TGCCGAC4600.020.33102655
Produced by FastQC (version 0.11.5)