FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689833
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.20004841751554361No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.14467269614529896No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT8690.12597251798623726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5500.048.6818162
GTATCAA25500.046.8435171
GTGGTAT6050.045.6710851
TATCAAC28950.041.5457732
TCAACGC31250.038.482424
CAACGCA31700.037.936145
ATCAACG31900.037.8315053
AACGCAG32750.036.7198686
GGTATCA13200.036.390491
ACGCAGA36050.033.3585437
CGCAGAG36300.033.1288078
GCAGAGT40050.030.4512639
CAGAGTA26750.026.52886410-11
GGGTTAC900.001708209723.6162471
AGAGTAC26300.022.53950710-11
AGAGTCG11200.022.3851475
ATCCCGC950.00234413822.365186
GAGTACG32100.022.10738612-13
GTACGGG33550.020.89861114-15
ATCGGCC1050.004188498520.235168
Produced by FastQC (version 0.11.5)