FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689833
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19590.28398177529923907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3850.032.1430241
CGGACTA350.00409403631.4355817
GTATCAA24950.031.1925491
ACGGAGA453.7890303E-430.5623719
TGGTATC4000.029.5497952
GGTATCA11400.028.4650631
TATCAAC27950.027.9307442
TCAACGC29200.025.9744474
ATCAACG29550.025.7616583
CAACGCA29900.025.5520155
AACGCAG30300.025.3072286
AGAGTAC29550.024.84244211
CGCAGAG30600.024.7896658
ACGCAGA30950.024.6869347
CAGAGTA30350.024.27644510
CCGAATT952.3092707E-623.16305714
GCAGAGT33000.022.910149
ACGGGGT4650.021.88659916
TACGGGG15000.021.63815715
AGTACGG33550.021.3966613
Produced by FastQC (version 0.11.5)