FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449623
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19570.43525353462789934No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT14540.3233820333924199No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13930.3098151117714174No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.286239805348036No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC12150.2702263896642298No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT11840.2633317245781466No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA10840.24109086946174904No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT10720.23842196684778139No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG10410.23152730176169814No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA10280.22863599059656645No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA9390.20884162954297267No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC8900.1979436105359379No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT8810.1959419335754621No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG8500.1890472684893789No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG8430.18749040863123106No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.1819301948521317No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT8140.18104056064747578No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA7890.1754803468683764No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA7490.16658400482181737No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA7310.16258065090086585No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC7180.15968933973573415No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT6970.15501876016129068No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG6940.15435153450779876No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT6740.14990336348451927No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT6720.1494585463821913No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC6550.1456776010124037No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC6320.1405622043356323No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA6080.1352243991076969No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC6000.1334451306983851No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA5920.1316658622890733No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA5860.13033141098208945No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG5570.12388156299833417No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG5500.12232470314018633No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG5370.11943339197505466No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT5180.11520762950293913No Hit
CTATTACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCACATGCAC5090.11320595254246334No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT4900.10898019007034782No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT4900.10898019007034782No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG4670.1038647933935764No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG4520.10052866512611676No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC4510.10030625657495279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6300.052.6116142
GTATCAA32150.046.6782461
GTGGTAT7400.045.9598961
TATCAAC37200.040.6662982
GACAGGG1650.038.6480981
GGTATCA14850.038.3618131
TCAACGC39450.038.239214
AACGCAG39500.038.0832256
CAACGCA39750.037.843715
ATCAACG40300.037.538123
ACGCAGA42450.035.5367937
CGCAGAG42700.035.328738
GCAGAGT44300.034.24469
GTCTAGG652.5463512E-432.7022361
AGGTGAC1251.6689228E-830.5956755
GACAGGT1551.3460522E-1030.1704481
CAGCTAG853.4895314E-529.995769
GCTTGTA2700.029.9164891
CTGTTCG1353.5270205E-828.329339
CGTCAGA1054.5213546E-628.329335
Produced by FastQC (version 0.11.5)